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For semaglutide, the prescribing information says steady-state exposure is reached after four to five weeks of once-weekly dosing

Post-translational cleavage of POMC by the proconvertases PC1/3 and PC2 successively generates ACTH, endorphins and the three MSH forms (alpha-, beta-, gamma-)

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Replacement with Trp 7 lowers the affinity (IC 50 : 0.26 nM3.3 nM) and strongly lowers the activity (EC 50 : 0.8 nM127 nM)

However, this dosing time-dependence is more common amongst drugs with shorter half-lives, such as less than 15 h, whereas oral semaglutide has a long half-life of approximately 1 week [1, 10]