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The size of individual spheroid was determined by measuring their optical areas using Image-J and by calculating the apparent radius ( r ) to estimate their apparent volume ( v ) according to the following formula: v =4/3 r 3
In each set, the time course is followed by the change from baseline (mean of days 3 to 1) to the mean of days 1-3, 7-9, and 18-20, as indicated
The escape latency was recorded and the mean of the four starting points were used for data analysis
May mask folate/B12 deficiency symptoms 5
Schulz R, Belosjorow S, Gres P, Jansen J, Michel MC, Heusch G: p38 MAP kinase is a mediator of ischemic preconditioning in pigs
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