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Gastroenterology 159 (3), 849863

This log helps you track your protocol, maintain site rotation, and identify patterns if problems arise

Pellieux C, Sauthier T, Domenighetti A, Marsh DJ, Palmiter RD, Brunner HR, Pedrazzini T

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Dose may be increased to 9 mg once daily if additional glycemic control is needed after at least 30 days on the 4 mg dose

For binding assays, 100 g of each purified fusion protein was first immobilized on 50 l of packed glutathione-agarose beads at room temperature for 1 hour, the beads were washed once with cold homogenization buffer B, then 300 l of the diluted cytosol was added and the beads tumbled for 2 h at 4 C