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Funding This study was supported by the Departments of Excellence 20182022 initiative of the Italian Ministry of Education, University and Research for the Department of Neuroscience, Imaging, and Clinical Sciences of the University of ChietiPescara

These metabolites were successfully separated and quantified using high-performance liquid chromatography (HPLC) equipped with UV-Vis detection and a reverse-phase C18 column, strictly following the protocol described by 2.6 Metabolic and biochemical profiling 2.6.1 Soluble carbohydrates To evaluate the reallocation of energy resources under Pb stress, soluble carbohydrates were extracted from the microalgal cultures using 80% (v/v) ethanol