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There are two ways to measure the B12 supply: directly measure B12 in the blood measure certain decomposition substances in the urine, which accumulate in the instance of deficiency According to both routes there are different methods

If you properly store the powder, it will retain its efficacy for quite some time

An increase in probiotic Bifidobacterium was detected in all donors (Jimnez-Arroyo et al., 2023)

Taking Neurobion Forte or similar products may provide some benefit if you have a mild deficiency

doi: 10.1016/S0022-2828(85)80130-9

That said, it's not a must-have product either, if you're pretty happy with the state of your skin