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Cells were diluted in fresh medium with a starting OD 600nm of 0.1 and allowed to grow at 30 C with shaking for several hours (46 h) until they reached log phase (OD 600nm 0.61.2)
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doi: 10.12998/wjcc.v4.i7.155 171 MuY.McManusD
The mechanisms by which certain medications bring about oral mucosal melanin hyperpigmentation include upregulation of tyrosinase activity, deposition of complexes of the drug with pre-existing melanin in the mucosa, or induction of local inflammatory reactions with the triggering of melanin production [11, 38]
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