dNTP Mix Machinery Since the methylated DNA (pUC19m)

Since the methylated DNA (pUC19m) contains a Nco I restriction site (the non-methylated (pUC19) DNA does not)

Cells of the ten microbes were mixed in a log-distributed abundance

The primer sets are designed to amplify a fragment of the supplied DNA from the respective species following bisulfite treatment

and methylation analysis

This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification

dNTP Mix Machinery Since the methylated DNA (pUC19m)dNTP Mix is a ready to use, ultra pure nucleotide mix that can be used to generate PCR amplicons using ZymoTaq, QuestTaq or other DNA polymerases.