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The cell powder was suspended in a 1:1 mixture of watersaturated phenol and extraction buffer (50 mM NaOAc and 10 mM Mg(OAc) 2 , pH 5.2) and vigorously stirred for 1 h at room temperature

However, because IGF-1 promotes cell growth, it could theoretically accelerate the progression of existing or dormant tumors

We offer both relative and absolute quantification strategies to track dynamic glutathionylation changes across sample groups

This study shows that MYC downregulation of mir-23a/b relieves repression of GLS as part of the oncogenic reprogramming of glutamine metabolism

10.1016/j.ctim.2018.06.002 89 HigashiY.MaruhashiT.NomaK.KiharaY

Cancer cells shift from normal OXPHOS to glycolysis metabolism to obtain a faster ATP production rate, maintain redox balance and synthesize nucleic acids, proteins and fatty acids