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One Nutrition Vitamin B12-Max oral spray 1200mcg (13.30 for 30ml, Morrisons) Six sprays gives 1200mcg of B12 = 36p per daily dose

In Control cells, nutlin-3 treatment down-regulated numerous genes involved in nucleotide metabolism, including DTYMK , CTPS1 , RRM1 , and RRM2 (Fig 3B)

After several PBS washes, cell pellets were resuspended in lysis buffer (1% SDS, 50 mM TrisHCl, pH 8, 10 mM EDTA)