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These modifications are designed to counteract the immunosuppressive tumor microenvironment (TME) by enhancing T-cell activity and survival, thereby directing CAR-T cells towards the tumor site

A well-known risk factor in humans is hypercholesterolemia, i.e., elevated total cholesterol (TC) and low-density lipoprotein cholesterol (LDL) (Pourrajab et al., 2021), and other important contributors to this disease include inflammation, oxidative stress and insulin resistance (Subramani et al., 2017)

Sterile water contains no preservative, so once the seal is punctured, its susceptible to contamination

Native chemical ligation in protein synthesis and semi-synthesis

typical therapeutic doses are well below the toxic threshold and are considered safe

Perform magnetic separation between each wash, remove supernatant and resuspend by gentle pipetting