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Nevertheless, use of metformin may decrease vitamin B 12 status: a meta-analysis of four short-term intervention trials (up to 4 months duration) in patients with type 2 diabetes found that metformin use decreased serum cobalamin concentrations by 57 pmol/L, which could have clinical implications if such patients have suboptimal vitamin B 12 status (146)

It is a small molecule made from three amino acids cysteine, glycine, and glutamate and it is produced naturally inside your cells

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For calcium-dependent aggregation assays, Syn WT (50 M) was pre-incubated with 3.0 mM CaCl for 30 min prior to initiating aggregation, as previously described 102