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The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)

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As a result, process engineers must systematically optimize several parameters during scale-up

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Step 5: 3D Printed Indirect Bonding Trays Once the setup is approved, a custom bonding tray is created