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DNA concentration was determined with a Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific) and Qubit fluorometer (Thermo Fisher Scientific)

For GTTs, animals were fasted for 4 h and a baseline blood glucose measurement was made prior to administering a 2 mg/kg i.p

Cycles : 8 weeks on/4 off (standard)

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Beyond 5 months: Full effect at maintenance dose The full appetite-suppressing effect usually becomes most noticeable after several months, once dosing is stable

Your baseline metabolism, influenced by age, muscle mass, and hormonal factors, also shapes how efficiently tirzepatide drives weight loss