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Activation of Glucagon-like Peptide-1 Receptor Inhibits Growth and Promotes Apoptosis of Human Pancreatic Cancer Cells in a cAMP-dependent Manner

We used murine Acly flox/flox hepatocellular carcinoma (HCC) cell lines and generated isogenic cell lines lacking ACLY or ACSS2 via adenoviral Cre treatment or CRISPR/Cas9 gene editing, respectively (Supplemental Figure 1A)

Strategies to minimize immunogenicity include careful peptide design to maintain similarity to natural sequences, appropriate formulation development, and monitoring for antibody formation during clinical development and post-market surveillance