7ps and Extended Data Fig
mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyski & Sacchis method (Chomczynski and Sacchi, 2006), following the manufacturers protocol
Pharmacol Biochem Behav 121:88101
NE neurons originate in the locus coeruleus (LC) of the brain and extend their axons through different regions such as the CNS cortex, hypothalamus, amygdala, cerebellum, and spinal cord to reach primary nerve centers or other neurons (Jovanovic et al., 2022)
However, acidification did not increase or decrease the expression of GluA1 in neurons [51]
N-acetylcysteine improves coronary and peripheral vascular function