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Shaking creates foam, introduces air bubbles into the solution, and can physically denature the peptide molecules
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We appreciate that those characteristics may sound rather technical
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To test the hypothesis that a disorder of ROS homeostasis is induced by OsG-produced oxidative stress, an antioxidant, GSH (50 mg/kg/ 6 h), was subcutaneously injected into animals with LOsG challenge as described above, defined as the LOsG.TdGSH group ( n = 10)
& Sullenger, B
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