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Particles in the subsets with high occupancy at the A-site were selected, re-extracted in a box size of 530 pixels (without rescaling, 0.8285 per pixel) and subjected to 3D refinement followed by per-particle CTF refinement, Bayesian polishing and second 3D refinement

The exact list of tested substances is not publicly disclosed in full detail

However, in scenarios where the FOXO4 is linked with the DRI peptide, it is suggested that this binding may not occur, potentially allowing p53 to interact with DNA and facilitate the continuation of the cell cycle toward programmed death