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Some researchers have used subcutaneous injection of scopolamine combined with a dry environment to establish DED mouse models, and immortalized human HCEC-2 cells were cultured under hypertonic conditions to establish hypertonic cell injury models and measure the levels of intracellular Fe 2+ , cellular ROS, and lipid peroxidation

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Journal of Neurochemistry 69, 1326-1329CrossRefGoogle Scholar 141Alam, Z.I., Jenner, A., Daniel, S.E

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Environmental factors, including diet and micronutrient availability, may influence disease penetrance and should therefore be considered when interpreting these findings