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Anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technology) HRP-conjugated at 1:5000 dilution were incubated with the membranes for 1 h at room temperature, and visualized using a chemiluminescence system

Front Plant Sci 10:1169 Weiland M, Mancuso S, Baluka F (2015) Signalling via glutamate and GLRs in Arabidopsis thaliana

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The goal is to minimize that as much as possible

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