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This flexibility and rapid manufacturability make lipid nanoparticle-mRNA formulations an important frontier in vaccine development, providing a platform that can be mobilized quickly against emerging infectious pathogens [82]

United European Gastroenterol J 9:781786

Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease

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In particular with the CRISPRa approach, the transcriptional activators that are fused to the dCas9 enzyme will increase the length of the construct, such that a combination of sgRNA and dCas9-transcriptional activators will be too large to load onto a single AAV vector