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Qualitative assessment of nitric oxide production by fluorescent dye 3-Amino-4-aminomethyl-2,7-difluorescein, diacetate (DAF-FM DA, Sigma) HUVECs grown in M199 based culture medium was stimulated with 1.0 g/ml BPC 157 for 30 min to induce the nitric oxide generation

Chen Y, Zeng X, Huang X, Serag S, Woolf CJ, Spiegelman BM (2017) Crosstalk between KCNK3-mediated ion current and adrenergic signaling regulates adipose thermogenesis and obesity

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