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Then the preparations were washed with 10% Phosphate Buffer Saline (PBS) 3 times for 5 minutes, dropped with Chromogen 3,3-diaminobenzidine for 510 seconds, washed with distilled water, counterstained with Hematoxylin Mayer for 510 seconds followed by washing with running tap water for 1015 minutes

While plasma EVs are infrequent under healthy conditions due to their limited ability to traverse the glomerular filtration barrier, uEVs play a crucial role in detecting renal damage and function as carriers for systemic markers [77]

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