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Subsequently, the cells were incubated with Cy5.5-labeled NP4 for varying time intervals of 1 h, 4 h, or 7 h, respectively
The cells were then washed with PBS, and GSH and GSSG levels were determined by applying the GSH/GSSG-Glo assay according to manufacturer instructions
Autopsy studies from severe COVID-19 cases revealed long-lasting transcriptomic alterations in the lungs, heart, kidney, liver and lymph nodes, consistent with sustained metabolic stress and incomplete recovery of homeostasis ( Building on this, we observed that human frontal cortex autopsy samples exhibit OXPHOS repression, HIF-1, immune and RAAS activation, and suppression of neuronal metabolic pathways ( Figure 4 )
Science 284:805808 Falcone E, Stellato F, Vileno B, Bouraguba M, Lebrun V, Ilbert M et al (2023) Revisiting the pro-oxidant activity of copper: interplay of ascorbate, cysteine, and glutathione
JP patent 1,228,473 Meister A, Anderson ME (1983) Glutathione
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