TUNEL assay INS-1 832/3 SNAP/FLAG-hGLP-1R cells seeded onto coverslips were incubated with or without 5 M thapsigargin in vehicle or 100 nM exendin-4-supplemented media for 8 hours before fixation and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay performed using the DeadEnd TM Fluorometric TUNEL system (Promega) following the manufacturers instructions
Available from: Accessed 1 Mar 2023 Brown E, Heerspink HJL, Cuthbertson DJ, Wilding JPH (2021) SGLT2 inhibitors and GLP-1 receptor agonists: established and emerging indications
Pills are often more shelf-stable and cheaper to manufacture
Similar cardiovascular outcomes in patients with diabetes and established or high risk for cor- onary vascular disease treated with dulaglutide with and without baseline metformin
It means escalating to 12mg because that is the top of the scale may not be necessary or even advisable if you are already at your target at 8mg
Inagaki N, Seino Y, Takeda J, et al