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(B) Enzyme-linked immunosorbent assay (ELISA) of interleukin-6 (IL-6) in supernatants from RAW264.7 cells treated with LPS in the presence or absence of Tir for 24 hours

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To improve the density quality of specific regions, this subset of particle projections was subjected to 3D auto-refinement with a mask on the complex, which was subsequently subjected to another round of 3D classifications with a mask on the ECD