The high hedonic value and motivational incentive of food are the main culprits for overeating or eating beyond the immediate metabolic need
This is one reason proper storage at 3646F (not at or below 32F) is critical
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56 Long-term overeating and a high-fat, Western-type diet have been shown in many studies to raise the risk of insulin resistance and obesity (particularly in the abdomen), as well as high cholesterol and triglyceride levels and high blood pressure
For the GFP tag knock-in construct, homology recombination templates were constructed with the following steps: (1) cloning a 2 kb genomic region of aptf-2 centered on the knock-in site into the pPD95.75 vector, (2) insertion of DNA encoding GFP and a linker of six amino acids (AGACCCAAGCTTGGTACC) between the last amino acid codon of aptf-2 and the stop codon, (3) the PAM site within the recombination template was mutated to prevent Cas9 cleavage
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