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The liver was removed, minced in DMEM and Hams F-12 medium (1:1) supplemented with 2 mM glutamine, 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 g/mL streptomycin and 1 mM sodium pyruvate (attachment medium) and the hepatocytes were filtered through a 100 m cell strainer

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Prins GS, Hu W-Y, Shi G-B, Hu D-P, Majumdar S, Li G, et al

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10.1007/s10067-016-3474-8 [DOI] [PMC free article] [PubMed] [Google Scholar] 378.Kydd, A