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Aliquots were spotted onto P81 phosphocellulose filters, which were then washed three times for 10 min each in 75 mM phosphoric acid, followed by a 5-min wash in methanol

The NHS confirms that taking up to 2mg (2000 micrograms) daily is unlikely to cause harm, with no established upper limit in the UK

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This is the most critical step

Polymorphisms in genes associated with collagen synthesis, muscle repair, and inflammation can influence an individuals susceptibility to ACL injuries