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The study on rhesus monkeys identified dose-dependent renal (kidney) toxicity as the primary limiting factor

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Common research protocols cited in the peptide community use 1 to 2 mg, 3 to 5 times weekly, over 8 to 12-week cycles

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The DNA region spanning from 2 569 pb to + 310 pb of CCL11 (GRCh38/hg38 chr17: 3428317334286052) was amplified by PCR from bisulfite-converted DNA using a primer set designed to account for cytosine conversion to uracil (Bisulfite Primer Design Tool, Zymo Research) (Additional file 2) and GoTaq DNA Polymerase (Promega, Madison, Wisconsin)