To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig
Your body produces glutathione on its own, but GSH levels can drop due to stress, poor diet, lack of sleep, or exposure to toxins and pollutants
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Free radicals are unstable molecules that damage cell membranes, proteins, and DNA, and antioxidants like glutathione are how the body keeps that damage in check
