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In cases of mild melanogenesis and non-pigmented forms, it is necessary to use a set of clinical and morphological criteria (localization, rate, and form of tumor growth), histological features (the combined directions of differentiation, correspondence between the cell type and their growth, the presence of appositional growth), detection of premelanin, and/or IHC study [37]

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Researchers have also explored whether or not PPD causes cancer

Cells were treated with 2 N HCl for 10 min at room temperature to achieve DNA denaturation, followed by a PBS wash and subsequent immunofluorescence staining as previously described

Bull Exp Biol Med 2006;141:3903