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Cells were cultured in Dulbeccos Modified Eagles medium (Welgene LM001-05) supplemented with 10% fetal bovine serum (Gibco 16000-044), and 1% penicillin/streptomycin (Gibco 15140-122)

J Biol Chem (1984) 259(15):977682

Temperature of the program was kept constant at 22C, and lights were set to turn on at 6 am and off at 6 pm to match the circadian cycle of the mouse

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