10.3389/fnbeh.2017.00035 Front
The more glutathione in circulation, the better the body is at combating oxidative stress and preventing the associated diseases
Microscopic images of scratch were taken and considered as time point zero
This week, were diving into three unsung heroes: AOD-9604 , Epitalon , and MOTS-c
GST pull-down vs immunoprecipitation Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads GST pull-downs are assays used to investigate protein-protein interactions
Quantification of mRNA was performed by using primers that span over two adjacent exons, quantified using the comparative standard curve method and normalized to hypoxanthine phosphoribosyltransferase ( Hprt ), a housekeeping gene
