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Lab Investig J Technol Methods Pathol 52:151158 Bagheri M, Moradi-Sharhrbabak M, Miraie-Ashtiani R, Safdari-Shahroudi M, Abdollahi-Arpanahi R (2016) Casecontrol approach application for finding a relationship between candidate genes and clinical mastitis in Holstein dairy cattle

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The inoculation volumes were adjusted to an initial OD 600 of 0.15 in 200 mL baffled Erlenmeyer flasks and cultured at 200 rpm in a shaking incubator (BR-43FL, TAITEC, Saitama, Japan) at 30 C