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Body weight was assessed daily just before the start of the dark cycle

After 72 h, the resulting solution was freeze-dried by using low-temperature freeze dryers, yielding P1(1.23 g) as a yellow powder

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Nevertheless, the target for GIPR antagonists is believed to be a neuronal population in the brain, as a peptideantibody conjugate that blocks GIPR while activating GLP-1R required central GIPR for maximal weight loss in DIO mice, as determined using mice lacking Gipr in cells expressing synapsin 12