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Sample or standard was added to GCL reaction buffer (400 mM Tris, 40 mM ATP, 20 mM L-glutamic acid, 2.0 mM EDTA, 20 mM sodium borate, 2 mM serine, and 40 mM MgCl 2 )

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The fat is then stored in the body, and the acetate is used for energy

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SL: Data curation, Investigation, Resources, Writing original draft