This isn't just a minor loss of potency we're talking about
To induce acute liver injury, 8-week-old NSI mice received two consecutive intraperitoneal injections of DMN (7 mg/kg, Sigma, 1.0% dissolved in saline)
Besides, it stimulated expression of early growth response 1 (egr-1) gene responsible for cytokine and growth factor generation and early extracellular matrix (collagen) formation (but also its repressor nerve growth factor 1-A binding protein-2 (nab2)) (44)
C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)
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Refrigerate reconstituted peptides