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Antibodies Specific primary antibodies: Akt (2532 S, 1:2000), pS473-Akt (9271 S, 1:1000), S6K (9202 S, 1:2000), pT389-S6K (9205 S, 1:1000), Stat5 (9420 S, 1:10000), and pY694-Stat5 (9356 S, 1:1000) were obtained from Cell Signaling

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9 Limitations of preclinical evidence Despite the growing body of preclinical evidence supporting the neuroprotective potential of astaxanthin, several important limitations should be acknowledged