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Surface plasmon resonance (SPR) spectroscopy The kinetics of the binding of the FBXO22 protein to either full-length BPC157 or single-site alanine-mutated BPC157 were assessed via an SPR assay on a Biacore T200 SPR system (Cytiva)

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The slow reaction between PfGrx C88S (S 2 ) and roGFP2 WT (SH) 2 was confirmed by electrophoretic mobility shift assays (Fig

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