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It is also important to mention that RNA and protein levels were determined by real-time PCR and Western blotting, respectively

doi: 10.3389/fnins.2015.00092 Received 07 February 2015 Accepted 05 March 2015 Published 20 March 2015 Volume 9 - 2015 Edited by Marc Claret, Institut d'Investigacions Biomdiques August Pi i Sunyer, Spain Reviewed by Joao Carlos Dos Reis Cardoso, University of Algarve, Portugal

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Numerous later studies have shown the association between increased plasma TMAO levels with a higher risk of cardiovascular events [63,64,65,66]

Overexpressing ACMSD reduces the NAD + level by dissipating ACMS from the de novo NAD + synthesis into the side branch pathway for acetyl-CoA production, while inhibiting ACMSD elevates NAD + concentration