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To this end, we transiently transfected HEK-293T cells with vectors encoding full-length wild type SnCE1 and truncated SnCE1 74-310 with a C-terminal myc-His 6 -tag and performed subcellular fractionation to reveal to which cellular compartments SnCE1 localizes and whether the autoproteolytic processing modulated by the SnCE1 acetylation state affects the localization of SnCE1

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These studies face methodological challenges, including the need to distinguish between reduction in binge episodes due to pharmacological effects on eating behavior versus secondary effects of nausea or gastrointestinal side effects

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The Ozempic prescribing information contains no body mass index eligibility criterion, because none of its three indications concerns weight