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Chen H, Malentacchi F, Fambrini M, Harrath AH, Huang H, Petraglia F

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The intrinsic pathway (mitochondrial pathway) involves two primary mechanisms of apoptosis: cytochrome c (Cyt C) release and the opening of the mitochondrial permeability transition pore (MPTP) (Taneja et al., 2001)

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injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )