The coupled resin was washed with PBS and remaining active groups were blocked by addition of 40 mL of 1 M ethanolamine, pH 9.0 and 5 h incubation at room temperature
Beyond 28 days, oxidative degradation and aggregation increase measurably
Frontiers then required the authors to resubmit the paper and undergo a seventh and eighth round of peer review, the comment states
Example calculations: 200mg vial + 2mL water = 100mg/mL (0.5mL injection delivers 50mg dose) 600mg vial + 3mL water = 200mg/mL (0.5mL injection delivers 100mg dose) 1000mg vial + 5mL water = 200mg/mL (1mL injection delivers 200mg dose) Most therapeutic protocols use 100200mg per injection, administered 23 times weekly
Typical research dosing for GHK-Cu is outlined in our dosage chart
At week 16, the primary end point of the trial, all patients were crossed over to open-label infliximab therapy through 50 weeks