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Butyrate promotes production of extra-thymic T reg cells in an intronic enhancer CNS1-dependent manner if administered systemically, but increases only intracolonic T reg cells production if administered locally via an enema ( reg cells in an FFAR2-dependent manner ( IL-10 is a key cytokine for T reg cells to exert anti-inflammatory effects

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133 Alternatively, a variety of screening tests which utilize hemolysate as a source of enzyme also can be used