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Several key sequence features underpin the triple receptor agonist activity of the molecule: Position 1 (Histidine) : The N-terminal histidine residue is critically important for glucagon receptor activation

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A key challenge in studying native GLP-1 is its extremely short in vivo half-life of approximately 12 minutes, owing to rapid inactivation by dipeptidyl peptidase-4 (DPP-4), which cleaves the N-terminal His-Ala dipeptide from GLP-1 to produce the inactive metabolite GLP-1(936)amide

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