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In fact, apart from ensuring direct access through the mucin, it was this recognition (of such a low 2-3uM solubility for niclosamide at neutral pH), that went into the initial thinking (see Supplemental Information S1 ) and prompted the invention and development of a niclosamide solution as opposed to any microparticle formulation that were all explored in the series of provisional patents (48)
Robertson, C
Sample metabolites were quantified using calibration curves made from the standards in Themo Chromeleon software, and further data processing to adjust for the relative quantities of metabolites in the standards and recovery of the internal standard (norvaline) was done in MS Excel
If your materials aren't stable and consistent, your results won't be either
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