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LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling

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Tirzepatide (Mounjaro, Zepbound) is a dual glucose-dependent insulinotropic polypeptide (GIP) and GLP-1 receptor agonist, representing a related but distinct medication class

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