For LC3 staining, the permeabilization step was additionally included, performed with a buffer containing 20 M digitonin (in 10% goat serum in PBS for 10 min, at RT)
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doi: 10.1016/j.jad.2008.12.012 22 MohammadMYHBushulaybiNAAlHumamASAlGhamdiAYAldakhilHAAlumairNAet al
They will understand the interactions, the monitoring requirements, and the nuances of optimizing both therapies simultaneously