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Chemiluminescent signals were developed with Luminata Forte HRP substrate (Merck Millipore) and observed using an ImageQuant LAS 350 machine (GE Healthcare)

There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes

2014 Mar;19(1):161-73

Your body has been sending you signals

If theres one vitamin that Id like all older adults and family caregivers to know more about, it would be vitamin B12