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Cells were resuspended in 5 to 10 mL of lysis buffer (20 mM sodium phosphate pH 7.5, 200 mM NaCl, 0.05% (w/v) Tween-20, 2 mM MgCl 2 , 0.5 mM TCEP, +400 U DNAse I, 12.5 mM imidazole and a tablet of Complete EDTA-free protease inhibitor cocktail, Roche, per 100 mL of buffer) per gram of cells

J Pineal Res (2010) 48(1):919

Opioid use disorder (OUD opioid addiction) is a chronic, medical disease, with serious potential consequences including disability, relapse, and death

The challenge is getting it into cells via supplementation

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